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Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...
Also, according to Bashiri, its expression levels can equal those of the T7 expression system. In addition to producing proteins, Xbrane also is developing its optiXpress service model.
Studier's T7 expression system uses the T7 promoter to "turn on" a gene of interest and the T7 RNA polymerase to transcribe that gene into messenger RNA (mRNA) so that E. coli ribosomes can use ...
Studier continued studying the T7 polymerase and promoter, fine-tuning the system for years and publishing new improved versions as recently as 2018.